Welcome
Welcome to the Course Website for EN.580.428 Genomic Data Visualization!
As the primary mode through which analysts and audience members alike consume data, data visualization remains an important hypothesis generating and analytical technique in data-driven research to facilitate new discoveries. However, if done poorly, data visualization can also mislead, bias, and slow down progress. This hands-on course will cover the principles of perception and cognition relevant for data visualization and apply these principles to genomic data, including large-scale single-cell and spatially-resolved omics datasets, using the R statistical programming language. Students will be expected to complete class readings, create weekly data visualizations as homework assignments, and make a major class presentation.
Course Information
Course Staff: Prof. Jean Fan and Caleb Hallinan
Lectures: 8:00am-9:50am Monday, Wednesday, and Friday. See Canvas for location details.
Office Hours: 10:00am-10:50am Monday, Wednesday, and by request. See Canvas for location details.
Course Details
☞ see Course tabFeatured Visualizations
Interrogating Spatial Spot Cluster Differential Gene Expression with 10x Visium
In these panels, I am depicting the representation of a 10x visium dataset in latent tSNE-embedded space and over the original spatial slide coordinates. I...
Identifying a Cluster of Breast Granular Cells
In the top left of my figure, I am depicting both my clusters made by kmeans clustering with k=7 in PCA space (with my cluster...
Homework 3: Differentially Expressed Genes analysis
[description] Those panels present a comprehensive visualization of Cluster 0 and its association with the gene SFRP4 through a combination of UMAP, spatial, and gene...
Spatial Transcriptomics Reveals a Distinct Epithelial Cell Population Defined by ELF3 Expression: A Multi-Dimensional Analysis of the Cluster in Interest
1. Describe your figure briefly so we know what you are depicting. Write a description to convince me that your cluster interpretation is correct.
Homework 2 submission
[description] In my visualization, I use points as the geometric primitive, angle and color for visual channel. The x-axis represents the PCA loadings for each...
Comparison of Scaled and Unscaled PCA: Gene Mean Expression, Variance, and PC1 Loadings
1. What data types are you visualizing? I am visualizing quantitative data, which includes log-transformed mean expression (x-axis), log-transformed variance (y-axis), and PC1 loading values...
Dimensionality Reduction using PCA
Homework 2
HW1: Gene expression pattern for GNB1 and HES4
1. What data types are you visualizing? I am visualizing HES4 and GNB1’s spatial gene expression patterns for eevee.
10 Genes with the Highest Counts are Expressed Across Most Spots
1. What data types are you visualizing? For this data visualization of the Eevee spatial transcriptomic data, I visualized both categorical data, the 10 genes...
Generation of Heatmap Expressing Top 20 Genes Within Pikachu Dataset
1. What data types are you visualizing? Within the Pikachu dataset that was visualized, gene expression levels across multiple individual cells proved to be a...
HW1: gene expression scatterplot
1. What data types are you visualizing? Spatial data of each cell, i.e the location of the cell within the section of the image, which...
All Visualizations
HW5: Identifying Cell Types and Tissue Structures in CODEX data
1. Figure Description. Figure A: 6 clusters in physical space. The axes represent x and y position. Figure B: 6 clusters in t-SNE space. The axes represent X1 and X2....
Identifying White Pulp Tissue Structure in CODEX Data
1. Figure Description and Interpretation I have performed quality control, dimensionality reduction using t-SNE, k-means clustering with optimal k=9 (from an elbow plot), and differential expression analysis on the CODEX...
Analysis of CODEX dataset
Based on the CODEX data, I hypothesize this tissue sample is taken from the white pulp region of the spleen, which is surrounded by red pulp. Some evidence/reasoning is outlined...
Analyzing Immune Cell Clusters in CODEX Dataset
Visualization Summary In this visualization, I analyzed two cell types within the CODEX dataset: T cells and B cells. First, the genes in the dataset were normalized, log-transformed, and clustered...
Identifying tissue structure in spleen tissue sample
1. Written Answer I decided to use techniques such as t-SNE and dimensionality reduction as well as normalizing the protein expression data to figure out the tissue structure in the...
Analysis of Cellular Clusters and Marker Expression in the CODEX Dataset
(A) This panel presents the visualization of cellular clusters in tSNE space, where distinct populations (Cluster 1 in red, Cluster 4 in green) are delineated against a background of unclustered...
Identification of White Pulp in CODEX Spleen Data Through Immune Cell Profiling
Based on the analysis of the CODEX dataset, I’m interpreting the tissue structure represented as the white pulp of the spleen. This conclusion is drawn from the identification of two...
Multi-Panel Data Visualization of CODEX Spleen Data
My analysis of the CODEX dataset aims to determine the tissue structure represented by the CODEX Spleen image by applying quality control, dimensionality reduction, K-means clustering, and differential expression analysis....
Identification of White Pulp Tissue Structure in Spleen CODEX Data
Here I perform clustering and differential expression analysis on a CODEX data set obtained from a spleen sample. Following an identification of the ideal 10 k-means clusters, I visualized the...
Clustering and Spatial Analysis of CODEX Tissue Types
Identifying White Pulp and Structural Fibroblast Populations in the Spleen
