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Reference-free cell-type deconvolution of multi-cellular spatially resolved transcriptomics data

View the Project on GitHub JEFworks-Lab/STdeconvolve

STdeconvolve

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STdeconvolve enables reference-free cell-type deconvolution of multi-cellular pixel-resolution spatially resolved transcriptomics data

The overall approach is now published in Nature Communications

Overview

STdeconvolve is an unsupervised machine learning approach to deconvolve multi-cellular pixel-resolution spatial transcriptomics datasets in order to recover the putative transcriptomic profiles of cell-types and their proportional representation within spatially resolved pixels without reliance on external single-cell transcriptomics references.

Example

library(STdeconvolve)
## load built in data
data(mOB)
pos <- mOB$pos
cd <- mOB$counts
annot <- mOB$annot
## remove pixels with too few genes
counts <- cleanCounts(cd, min.lib.size = 100)
## feature select for genes
corpus <- restrictCorpus(counts, removeAbove=1.0, removeBelow = 0.05)
## choose optimal number of cell-types
ldas <- fitLDA(t(as.matrix(corpus)), Ks = seq(2, 9, by = 1))
## get best model results
optLDA <- optimalModel(models = ldas, opt = "min")
## extract deconvolved cell-type proportions (theta) and transcriptional profiles (beta)
results <- getBetaTheta(optLDA, perc.filt = 0.05, betaScale = 1000)
deconProp <- results$theta
deconGexp <- results$beta
## visualize deconvolved cell-type proportions
vizAllTopics(deconProp, pos,
             groups = annot, 
             group_cols = rainbow(length(levels(annot))),
             r=0.4)	  

More details can be found in the tutorials.

Tutorials

Installation

To install STdeconvolve, we recommend using remotes:

require(remotes)
remotes::install_github('JEFworks-Lab/STdeconvolve')

STdeconvolve is also now available through Bioconductor.

Note that through Bioconductor (release 3.15), the R version must be >=4.2.

if (!require("BiocManager", quietly = TRUE))
    install.packages("BiocManager")

# The following initializes usage of Bioc devel
BiocManager::install(version='devel')

BiocManager::install("STdeconvolve")

Contributing

We welcome any bug reports, enhancement requests, general questions, and other contributions. To submit a bug report or enhancement request, please use the STdeconvolve GitHub issues tracker. For more substantial contributions, please fork this repo, push your changes to your fork, and submit a pull request with a good commit message.

Reproducing Analyses

Links below point to code outlining the preprocessing of datasets used for analyses in the manuscript.